The goal of this project is to mainstream biodiversity, nature-based solutions and ecosystem management, seek recognition for these as cross-sectoral tasks and integrated them in subnational Biodiversity Strategy and Action Plans and sector plans in three model city-regions. ICLEI launched the four-year INTERACT-Bio project in three Global South countries: Brazil, India and Tanzania. ICLEI is using a co-creation and co-production approach to implement the project activities. Through the project, city-regions will align their planning with their National Biodiversity Strategy and Action Plans (NBSAPs), which are required by the Convention on Biological Diversity (CBD). Through strengthened cooperation between the different levels of government, subnational action in support of the NBSAPs is promoted and enabled. Such collaborative approaches will ultimately support nations to accelerate biodiversity goals.